Installed and working Host platform: Windows 10 64-bits Hypervisor (Type 2): VirtualBox 6.1 (configuration as below) Guest: MS-DOS 6.22 English US (ENU) More information here . Download MS-DOS 6.22 from here . Steps Install VirtualBox from https://www.virtualbox.org Create Guest using the same parameters as the configuration file below. Install MS-DOS VirtualBox configuration file /cygdrive/f/VMs, Emulators, Simulators/VirtualBox VMs/MS-DOS/Microsoft MS-DOS 6.22 Microsoft MS-DOS 6.22.vbox <?xml version="1.0"?> <!-- ** DO NOT EDIT THIS FILE. ** If you make changes to this file while any VirtualBox related application ** is running, your changes will be overwritten later, without taking effect. ** Use VBoxManage or the VirtualBox Manager...